发布时间:*开通会员可解锁* 14:45:43
截止时间:*开通会员可解锁* 09:42:50
申购主题:大肠杆菌
送货时间:合同签订后7天内送达
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| 序号 | 采购内容 | 数量/单位 | 预算单价 | 品牌 | 型号 | 规格参数 | 质保及售后服务 | 附件 |
| 1 | 两个菌株 | 1.0 | *开通会员可解锁*.00 | 不限 | 不限 | 1. 该菌株用于表达IKZF2-ZF2蛋白,相关文献信息如下: IKZF2 zinc finger 2 domain (IKZF2-ZF2, Uniprot accession number Q9UKS7,residues 136-164) was cloned into a pET21a vector containingan N-terminal 6His-GST tag containing a TEV protease site. IKZF2-ZF2 was expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells using LB media supplemented with 50 uM ZnCl. 2. 该菌株用于表达CRBNmidi蛋白,该蛋白不包括MBP标签,相关文献信息如下:The stabilizing mutations in human CRBN (UniProt: Q96SW2) were designed using the PROSS server accessed in February 2022. DNA encoding the CRBN constructs was synthesized as gBlock Gene Fragments by Integrated DNA Technologies and inserted into a pNIC28-Bsa4 (Addgene #26103) vector using ligation-independent cloning, aswell as into a pNIC28-Bsa4 with an additional maltose-binding protein(MBP) tag inserted between the His, and TEV (Tobacco Etch Virus)protease cleavage site. The final CRBNmidi construct contained amino acids 41-187 followed by a Gly-Ser-Gly linker and amino acids 249-426 with single-point mutations C78l, I92V, K116N, Q134E, R283W,C287N,V2935,G302D,L342R, C343E, T359I and L423I. 3.需求方的实验方案是拿到该菌株后,进行杆菌培养、杆菌裂解及蛋白纯化操作。如有说不到的信息,欢迎再沟通确认。*开通会员可解锁* 李 | 质保期:_____1_年,产品的质量应符合国家相关质量标准。 |